6NBC
human methemoglobin state 1 determined using single-particle cryo-EM at 200 keV
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.8 |
Number of Particle | 1232543 |
Software | RELION,PHENIX,Leginon,CTFFIND,UCSF Chimera |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |