6NAV
Cryo-EM reconstruction of Sulfolobus islandicus LAL14/1 Pilus
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.1 |
| Software | EMAN2,SPIDER,RELION,Rosetta,PHENIX,Coot,EPU,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
