6NAK
BACTERIAL PROTEIN COMPLEX TM BDE complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.981 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.310, 113.990, 177.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.400 - 3.140 |
| R-factor | 0.19772 |
| Rwork | 0.192 |
| R-free | 0.30166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fpe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.671 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 3.300 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.169 | |
| Number of reflections | 30297 | 1505 |
| <I/σ(I)> | 7.47 | |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 4.56 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 13 % PEG 8000, 1 mM Imidazole pH 7 |
