6N99
Xylose isomerase 2F1 variant from Streptomyces sp. F-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.458 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.776, 82.293, 140.313 |
Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
Resolution | 19.757 - 2.800 |
R-factor | 0.269 |
Rwork | 0.267 |
R-free | 0.31630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s5n |
RMSD bond length | 0.003 |
RMSD bond angle | 0.519 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((dev_3139: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 37206 | |
<I/σ(I)> | 5.57 | |
Completeness [%] | 97.9 | |
Redundancy | 3.047 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 2.0 M lithium sulfate 8% 2-methyl-2,4-pentanediol 0.1 M imidazole pH 8.5 |