6N98
Xylose isomerase 1F1 variant from Streptomyces sp. F-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.458 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 87.100, 92.900, 99.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.770 - 1.550 |
R-factor | 0.1936 |
Rwork | 0.193 |
R-free | 0.21440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s5n |
RMSD bond length | 0.006 |
RMSD bond angle | 0.817 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((dev_3139: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 52732 | |
<I/σ(I)> | 18.08 | |
Completeness [%] | 89.8 | |
Redundancy | 12.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M bis-tris propane pH 7.0 2.5 M ammonium sulfate |