6N8X
Hsp90-alpha bound to PU-11-trans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.782, 91.251, 97.857 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.813 - 1.495 |
R-factor | 0.160476964783 |
Rwork | 0.159 |
R-free | 0.18495 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.061 | 0.378 |
Number of reflections | 48469 | |
<I/σ(I)> | 42.4 | 3.42 |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 7.1 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Na Cacodylate pH 6.5, MgCl2, PEG 2000 MME. Incubate with PU-11-trans prior to setup. |