6N7J
BDBV223 Fab bound to synthetic peptide of Bundibugyo virus Glycoprotein Stalk
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 70 |
Detector technology | PIXEL |
Collection date | 2016-05-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.452, 191.580, 63.362 |
Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
Resolution | 48.356 - 3.684 |
R-factor | 0.2289 |
Rwork | 0.228 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6MAM chains H & L |
RMSD bond length | 0.003 |
RMSD bond angle | 0.679 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.356 | 3.820 |
High resolution limit [Å] | 3.680 | 3.680 |
Rmerge | 0.310 | 0.680 |
Rpim | 0.190 | 0.430 |
Number of reflections | 12104 | 1176 |
<I/σ(I)> | 3.7 | |
Completeness [%] | 97.1 | 96.4 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.950 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 20% w/v PEG8000, 0.2 M magnesium acetate, 0.1 M sodium cacodylate:HCl, pH 6.5 |