6N73

Crystal Structure of ATPase delta1-79 Spa47 R271A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.550
Spacegroup name	P 1 21 1
Unit cell lengths	44.199, 154.117, 54.905
Unit cell angles	90.00, 110.03, 90.00

Refinement procedure

Resolution	32.295 - 2.398
R-factor	0.1874
Rwork	0.185
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5swj
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.111	0.079	0.652
Rmeas	0.123	0.087	0.732
Rpim	0.050	0.036	0.326
Total number of observations	150622
Number of reflections	25991	2679	2430
<I/σ(I)>	7.7
Completeness [%]	96.4	97.6	90.6
Redundancy	5.8	5.9	4.5
CC(1/2)		0.995	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	Tris, Ammonium Acetate, Lithium Sulfate, PEG 4000, MPD