6N72

Crystal Structure of ATPase delta1-79 Spa47 E267R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	P 1 21 1
Unit cell lengths	43.965, 153.823, 54.793
Unit cell angles	90.00, 109.90, 90.00

Refinement procedure

Resolution	41.339 - 2.735
R-factor	0.1956
Rwork	0.193
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5swj
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.750	5.920	2.750
Rmerge	0.248	0.109	1.225
Rmeas	0.268	0.118	1.326
Rpim	0.101	0.044	0.501
Total number of observations	121252
Number of reflections	17714	1802	1708
<I/σ(I)>	2.6
Completeness [%]	98.6	98.1	99.2
Redundancy	6.8	6.8	6.9
CC(1/2)		0.993	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	Tris, Ammonium Acetate, Lithium Sulfate, PEG 4000, MPD