6N6N
FtsY-NG high-resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.420, 76.883, 106.935 |
| Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
| Resolution | 36.171 - 1.877 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cqp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.171 | 1.944 |
| High resolution limit [Å] | 1.877 | 1.877 |
| Rmerge | 0.053 | |
| Rmeas | 0.074 | |
| Rpim | 0.053 | |
| Number of reflections | 46635 | |
| <I/σ(I)> | 11.02 | |
| Completeness [%] | 99.5 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 297.15 | 0.1 M Bis-Tris, 26% PEG 3350, 215 mM Ammonium acetate, pH 5.8 |
