6N6M

Crystal Structure of ATPase delta1-79 Spa47 R189A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	43.926, 154.070, 54.916
Unit cell angles	90.00, 109.65, 90.00

Refinement procedure

Resolution	39.952 - 2.788
R-factor	0.1845
Rwork	0.182
R-free	0.24090
Starting model (for MR)	5swj
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.188	0.084	1.276
Rmeas	0.204	0.091	1.395
Rpim	0.078	0.034	0.552
Total number of observations	108054
Number of reflections	16542	1700	1635
<I/σ(I)>	3
Completeness [%]	96.5	96.9	94.8
Redundancy	6.5	7	6
CC(1/2)		0.995	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	Tris, Ammonium Acetate, Lithium Sulfate, PEG 4000, MPD