6N6H
Vibrio cholerae Oligoribonuclease bound to pCpU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.070, 89.070, 60.143 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.791 - 1.757 |
| R-factor | 0.1617 |
| Rwork | 0.161 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6a |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.430 | 47.430 | 1.850 |
| High resolution limit [Å] | 1.757 | 5.550 | 1.760 |
| Rmerge | 0.035 | 0.986 | |
| Rmeas | 0.062 | 0.037 | 1.030 |
| Rpim | 0.018 | 0.011 | 0.297 |
| Total number of observations | 334350 | ||
| Number of reflections | 27804 | 964 | 4001 |
| <I/σ(I)> | 21.8 | 13.6 | 0.8 |
| Completeness [%] | 99.9 | 99.9 | 99.6 |
| Redundancy | 12 | 11.3 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol |
