6N6G

Vibrio cholerae Oligoribonuclease bound to pCG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 32 2 1
Unit cell lengths	88.976, 88.976, 59.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.716 - 2.018
R-factor	0.1629
Rwork	0.162
R-free	0.18690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n6a
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.295	47.295	2.130
High resolution limit [Å]	2.018	6.380	2.020
Rmerge		0.027	1.162
Rmeas	0.087	0.028	1.216
Rpim	0.025	0.008	0.353
Total number of observations	219716
Number of reflections	18345	647	2621
<I/σ(I)>	20.6	21.3	0.7
Completeness [%]	99.9	99.9	99.4
Redundancy	12	11	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol