6N6E

Vibrio cholerae Oligoribonuclease bound to pGA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 32 2 1
Unit cell lengths	89.615, 89.615, 59.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.311 - 1.578
R-factor	0.1524
Rwork	0.152
R-free	0.15880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n6a
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.311	47.311	1.660
High resolution limit [Å]	1.578	4.990	1.580
Rmerge		0.036	0.938
Rmeas	0.064	0.039	1.000
Rpim	0.022	0.014	0.340
Total number of observations	319761
Number of reflections	38266	1311	5473
<I/σ(I)>	17.8	14.1	0.8
Completeness [%]	99.8	99.8	98.7
Redundancy	8.4	7.8	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol