6N6C
Vibrio cholerae Oligoribonuclease bound to pAA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.375, 89.375, 59.451 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.750 - 1.619 |
| R-factor | 0.1466 |
| Rwork | 0.146 |
| R-free | 0.15460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.148 | 47.148 | 1.710 |
| High resolution limit [Å] | 1.619 | 5.120 | 1.620 |
| Rmerge | 0.023 | 1.060 | |
| Rmeas | 0.049 | 0.024 | 1.110 |
| Rpim | 0.014 | 0.007 | 0.326 |
| Number of reflections | 35203 | 1210 | 5051 |
| <I/σ(I)> | 28.1 | 23.6 | 0.7 |
| Completeness [%] | 99.9 | 99.9 | 99.3 |
| Redundancy | 12 | 11.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol |
