6N62

Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91980
Spacegroup name	P 41 21 2
Unit cell lengths	173.771, 173.771, 388.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.883 - 3.803
R-factor	0.2872
Rwork	0.286
R-free	0.33360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljz
RMSD bond length	0.003
RMSD bond angle	0.738
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.940
High resolution limit [Å]	3.800	3.800
Rpim	0.032	1.554
Number of reflections	59257	5662
<I/σ(I)>	12.34	0.59
Completeness [%]	100.0	100
Redundancy	11.4	8.5
CC(1/2)	1.000	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	295	0.1 M HEPES, pH 6.8, 0.2 M magnesium chloride, 7% w/v PEG3350, 4% v/v glycerol, 4% v/v ethylene glycol