6N5Z

Crystal structure of the SNX5 PX domain in complex with the Sema4C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	42.520, 87.698, 100.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.596 - 2.450
R-factor	0.2297
Rwork	0.225
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.333
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.600	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	14370
<I/σ(I)>	14.2
Completeness [%]	99.5
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	25% PEG3350, 0.1 M Tris (pH 8.0), 5% Cymal-7