6N5Y
Crystal structure of the SNX5 PX domain in complex with the CI-MPR (space group P212121 - Form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97803 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 33.063, 134.584, 98.266 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.260 |
R-factor | 0.2116 |
Rwork | 0.209 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.838 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 10740 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 99.8 | |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 75% of 0.2 M (NH4)SO4, 0.1 M MES pH 6.5, 30% (w/v) PEG5000 MME + 15% glycerol + 30% v/v (+/-)-2-Methyl-2,4-pentanediol |