6N5S
Structure of Human pir-miRNA-320b-2 Apical Loop and One-base-pair Stem Fused to the YdaO Riboswitch Scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9202 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 114.560, 114.560, 115.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.137 - 2.802 |
R-factor | 0.1789 |
Rwork | 0.176 |
R-free | 0.20080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qk8 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.137 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.062 | 1.730 |
Rpim | 0.020 | 0.499 |
Number of reflections | 21893 | |
<I/σ(I)> | 23 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 9.92 | 9.95 |
CC(1/2) | 0.998 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 1.9 M (NH4)2SO4, 0.2 M Li2SO4, and 0.1 M HEPES pH 7.4 |