6N5K

Structure of Human pir-miRNA-449c Apical Loop and One-base-pair Fused to the YdaO Riboswitch Scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	113.960, 113.960, 114.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.847 - 3.098
R-factor	0.1778
Rwork	0.177
R-free	0.18640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qk8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.847	3.180
High resolution limit [Å]	3.098	3.098
Rmeas	0.112	2.330
Rpim	0.019	0.444
Number of reflections	16089	16089
<I/σ(I)>	26.6	1.8
Completeness [%]	100.0	100
Redundancy	39.8	39.2
CC(1/2)	1.000	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	297	Magnesium chloride, Cyclic di-AMP, Potassium Chloride, HEPEs