6N5J

FtsY-NG high-resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9536
Spacegroup name	P 1 21 1
Unit cell lengths	34.784, 76.104, 108.704
Unit cell angles	90.00, 93.95, 90.00

Refinement procedure

Resolution	44.160 - 1.370
R-factor	0.1411
Rwork	0.139
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.895
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.160	1.419
High resolution limit [Å]	1.370	1.370
Rmerge	0.018	0.172
Rpim	0.018	0.172
Number of reflections	118474
<I/σ(I)>	16.9	4.14
Completeness [%]	99.9	99.7
Redundancy	2	2
CC(1/2)	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297.15	0.1 M Bis-Tris pH 5.8, 215 mM ammonium acetate, %20 PEG 3350