6N5I
FtsY-NG high-resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.753, 76.207, 108.989 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 34.670 - 1.498 |
| R-factor | 0.1352 |
| Rwork | 0.133 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 1.552 |
| High resolution limit [Å] | 1.498 | 1.498 |
| Rmerge | 0.023 | 0.132 |
| Rmeas | 0.032 | 0.186 |
| Rpim | 0.023 | 0.132 |
| Number of reflections | 88464 | |
| <I/σ(I)> | 18.22 | |
| Completeness [%] | 97.3 | 96.78 |
| Redundancy | 1.8 | 1.7 |
| CC(1/2) | 0.999 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 297.15 | 0.1 M Bis-Tris pH 5.8, 26.5% PEG 2000 MME, 175 mM KBr |
