6N5E

Broadly protective antibodies directed to a subdominant influenza hemagglutinin epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9787
Spacegroup name	P 31
Unit cell lengths	153.560, 153.560, 91.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.329 - 3.000
R-factor	0.257
Rwork	0.249
R-free	0.28720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1HA0 6n5b & 4HKX
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.329	50.000	3.300
High resolution limit [Å]	3.000	44.329	3.000
Rmerge	0.321		1.760
Rmeas	0.350		1.912
Total number of observations	311466
Number of reflections	48395	0	12035
<I/σ(I)>	7.28		1.36
Completeness [%]	99.8		99.6
Redundancy	6.436		6.482
CC(1/2)	0.984		0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M ammonium sulfate, 0.1 M MES, pH 6.5, 20% w/v PEG8000