6N5B

Broadly protective antibodies directed to a subdominant influenza hemagglutinin epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	124.002, 124.002, 90.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.124 - 3.500
R-factor	0.2377
Rwork	0.235
R-free	0.27840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.124	46.124	3.080
High resolution limit [Å]	3.000	13.430	3.000
Rmerge	0.156	0.028	6.146
Rmeas	0.174	0.032	6.849
Total number of observations	83144
Number of reflections	10398	199	1165
<I/σ(I)>	6.1	35.12	0.13
Completeness [%]	98.8	92.6	98.6
Redundancy	5.158	4.814	5.219
CC(1/2)	0.998	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES, pH 6.5, 12% PEG20000