6N5A
Crystal structure of an equine H7 hemagglutinin from A/equine/NY/49/73 (H7N7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 87.404, 87.404, 524.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.240 - 3.300 |
| R-factor | 0.20181 |
| Rwork | 0.200 |
| R-free | 0.23738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bsg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.200 | 1.000 |
| Rpim | 0.050 | 0.370 |
| Number of reflections | 19888 | 961 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 15.7 | 9.8 |
| CC(1/2) | 0.990 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES, pH 6.5, 2.4 M ammonium sulfate |
