6N53
Crystal structure of human uridine-cytidine kinase 2 complexed with 2'-azidouridine monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 198 |
Detector technology | PIXEL |
Collection date | 2018-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 89.896, 142.404, 248.566 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.818 - 2.700 |
R-factor | 0.2003 |
Rwork | 0.196 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uei |
RMSD bond length | 0.002 |
RMSD bond angle | 0.755 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmeas | 0.084 | 0.492 |
Rpim | 0.028 | 0.195 |
Number of reflections | 21912 | 958 |
<I/σ(I)> | 29.3 | 1.85 |
Completeness [%] | 98.0 | 85.5 |
Redundancy | 8 | 5.3 |
CC(1/2) | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Na/K phosphate, 22% PEG 3350 |