6N4A

PII-like SbtB from Cyanobium sp PCC 7001 (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.953657
Spacegroup name	P 1
Unit cell lengths	46.622, 51.030, 66.598
Unit cell angles	94.39, 106.13, 103.55

Refinement procedure

Resolution	49.030 - 2.300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	24704	2386
<I/σ(I)>	15.2
Completeness [%]	98.0	95.2
Redundancy	3.8	3.8
CC(1/2)		0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	25 mM Na2H/KH2PO4 20% PEG 3350