6N40
Crystal structure of MmpL3 from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2018-04-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.239, 130.917, 155.633 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.185 - 3.307 |
R-factor | 0.2684 |
Rwork | 0.266 |
R-free | 0.31450 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.631 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.185 | 100.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.110 | 3.300 |
Rmerge | 0.145 | 0.061 | 2.870 |
Rmeas | 0.155 | 0.065 | 3.067 |
Rpim | 0.054 | 0.024 | 1.058 |
Total number of observations | 220075 | ||
Number of reflections | 26284 | 2795 | 2574 |
<I/σ(I)> | 5 | ||
Completeness [%] | 94.0 | 99 | 99.8 |
Redundancy | 8.4 | 7.8 | 8 |
CC(1/2) | 0.997 | 0.574 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20%PEG400 and 0.1M LiCl |