Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.984 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 135.822, 145.712, 148.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.300 - 3.000 |
| R-factor | 0.2453 |
| Rwork | 0.243 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OP3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.484 |
| Number of reflections | 29134 | 1346 |
| <I/σ(I)> | 27.2 | 3.1 |
| Completeness [%] | 97.5 | 89.9 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 25% Peg 400, 0.2M imidazole malate |
