6N2V
Manganese riboswitch from Xanthmonas oryzae bound to Mn(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.769 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.296, 85.167, 92.463 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.640 - 2.850 |
R-factor | 0.2102 |
Rwork | 0.209 |
R-free | 0.22070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y1i |
RMSD bond length | 0.008 |
RMSD bond angle | 0.497 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.640 | 2.952 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.118 | 2.932 |
Rmeas | 0.127 | 3.150 |
Rpim | 0.045 | 1.131 |
Number of reflections | 15512 | 1510 |
<I/σ(I)> | 12.85 | 0.66 |
Completeness [%] | 99.8 | 99.07 |
Redundancy | 7.7 | 7.6 |
CC(1/2) | 0.999 | 0.306 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 mM RNA in 10 mM Na Cacodylate pH 7, 50 mM NaCl, 10 mM MgCl2, mixed in a 1:1 ratio with 10% MPD, 40 mM Na Cacodylate pH 7, 12 mM spermine tetrahydrochloride, 80 mM SrCl2, 20 mM MgCl2. |