6N2O
2-oxoglutarate:ferredoxin oxidoreductase from Magnetococcus marinus with 2-oxoglutarate, coenzyme A and succinyl-CoA bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.407, 100.532, 202.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.006 - 2.824 |
R-factor | 0.2148 |
Rwork | 0.212 |
R-free | 0.26050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6n2n |
RMSD bond length | 0.003 |
RMSD bond angle | 0.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 38629 | 2935 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 90.7 | |
Redundancy | 8.1 | |
CC(1/2) | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | 27% (w/v) PEG 4000 and 0.08 M sodium cacodylate pH 5.5 |