6N2N
Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from Magnetococcus marinus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.282, 123.161, 162.487 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 98.152 - 1.937 |
R-factor | 0.1698 |
Rwork | 0.168 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wbx 5c4i 3g2e |
RMSD bond length | 0.005 |
RMSD bond angle | 0.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.010 |
High resolution limit [Å] | 1.937 | 1.937 |
Number of reflections | 125663 | 11651 |
<I/σ(I)> | 7.7 | |
Completeness [%] | 97.6 | |
Redundancy | 4 | |
CC(1/2) | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | 14% (w/v) PEG 8000, 0.21-0.28 M (NH4)2SO4, and 0.10 M MES pH 6.5 |