6N2I
Lon protease AAA+ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-06-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 65 |
| Unit cell lengths | 88.600, 88.600, 67.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.300 - 3.500 |
| R-factor | 0.2866 |
| Rwork | 0.284 |
| R-free | 0.32540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ypl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.661 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.10.2155) |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 4.400 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 3856 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% (w/v) PEG 400, 0.2 M Lithium sulfate, 0.1 M sodium Cacodylate |
