6N2H
Structure of D-ornithine/D-lysine decarboxylase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.740, 52.280, 86.700 |
| Unit cell angles | 90.00, 127.60, 90.00 |
Refinement procedure
| Resolution | 46.880 - 1.720 |
| R-factor | 0.1647 |
| Rwork | 0.164 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qgh |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 1.782 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.163 | 2.555 |
| Rmeas | 0.176 | 2.764 |
| Rpim | 0.067 | 1.044 |
| Number of reflections | 50643 | 5022 |
| <I/σ(I)> | 6.92 | 0.83 |
| Completeness [%] | 99.9 | 99.82 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.994 | 0.247 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05 M potassium phosphate, pH 7, 28-30% dioxane |
