6N2C
The Crystal Structure of Caldicellulosiruptor hydrothermalis Tapirin C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-03 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.160, 90.430, 158.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.000 - 1.750 |
| R-factor | 0.16896 |
| Rwork | 0.168 |
| R-free | 0.21066 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.008 |
| Data reduction software | PROTEUM PLUS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 111011 | 16854 |
| <I/σ(I)> | 19.6 | 2.08 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 8.47 | 6.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5 mM to 35 mM zinc acetate and 15% to 24% w/v PEG 3350 |
