6N2B
The Crystal Structure of Caldicellulosiruptor kristjanssonii Tapirin C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-21 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.093, 98.317, 196.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.425 - 2.650 |
R-factor | 0.186 |
Rwork | 0.183 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wa0 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.986 |
Data reduction software | PROTEUM PLUS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 38585 | 3969 |
<I/σ(I)> | 4.89 | 1.03 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.23 | 4.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M citric acid pH 3.0 to 4.0 and 10% to 15% w/v polyethylene glycol 3350 |