6N1Z

Importin-9 bound to H2A-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97938
Spacegroup name	P 21 21 2
Unit cell lengths	127.424, 223.289, 131.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.440 - 2.700
R-factor	0.21
Rwork	0.209
R-free	0.23800
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.094	2.086
Number of reflections	103239
<I/σ(I)>	7
Completeness [%]	97.7	98.9
Redundancy	10.1	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.04 M MES, 0.11 M potassium acetate, 2 mM magnesium acetate, 2 mM DTT, 3.0 M potassium formate, 25% glycerol