6N1Y
Structure of L509V CAO1 - growth condition 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 101.210, 101.210, 450.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.370 - 2.150 |
| R-factor | 0.19884 |
| Rwork | 0.198 |
| R-free | 0.21832 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5u8x |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.170 | 2.535 |
| Number of reflections | 146769 | 23282 |
| <I/σ(I)> | 10 | 1.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 12.9 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281.15 | 33% w/v sodium polyacrylate 0.1 M HEPES pH 6 |
