6N1L
The complement inhibitory domain of B. burgdorferi BBK32.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2017-11-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.973 |
Spacegroup name | P 65 |
Unit cell lengths | 66.530, 66.530, 79.508 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.260 - 1.722 |
Rwork | 0.205 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j0k |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmeas | 0.090 | |
Rpim | 0.019 | 0.397 |
Number of reflections | 21152 | 2091 |
<I/σ(I)> | 35.25 | 1.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 22.1 | 16.8 |
CC(1/2) | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1M MES (pH 6.5), 0.2M ammonium sulfate, 30% PEG-MME 5,000 |