6N1G
Crystal structure of Aquaglyceroporin AQP7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-09 |
Detector | DECTRIS PILATUS 6M-F |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 385.658, 385.658, 385.658 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.711 - 3.995 |
R-factor | 0.2327 |
Rwork | 0.232 |
R-free | 0.27660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d9s |
RMSD bond length | 0.007 |
RMSD bond angle | 1.029 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3235: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.711 | 4.137 |
High resolution limit [Å] | 3.995 | 3.995 |
Number of reflections | 18776 | |
<I/σ(I)> | 38.77 | |
Completeness [%] | 88.0 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 30% w/v polyethylene glycol 400, 100 mM MOPS pH 7.0, 100 mM NaCl. |