6N0T
tRNA ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.310, 56.688, 172.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.416 - 2.511 |
R-factor | 0.185 |
Rwork | 0.178 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nov |
RMSD bond length | 0.007 |
RMSD bond angle | 1.008 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.044 | 0.436 |
Number of reflections | 17102 | |
<I/σ(I)> | 21.4 | 2.5 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 7.6 | 5.4 |
CC(1/2) | 0.991 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M HEPES pH 7.6 and 2.5 M ammonium sulfate |