6N08

Crystal structure of hemagglutinin from influenza virus A/Netherlands/209/1980 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-10-02
Detector	NOIR-1
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	201.990, 188.770, 108.760
Unit cell angles	90.00, 109.82, 90.00

Refinement procedure

Resolution	46.344 - 1.916
R-factor	0.2253
Rwork	0.224
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.344	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	292124	14353
<I/σ(I)>	5.6	0.1
Completeness [%]	97.4	97
Redundancy	6.7	6.2
CC(1/2)	0.994	0.264

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M Hepes pH 7.5, 2.0M ammonium sulfate