6N05

Structure of anti-crispr protein, AcrIIC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	105
Detector technology	CCD
Collection date	2016-07-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.9789
Spacegroup name	P 43 21 2
Unit cell lengths	71.880, 71.880, 135.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.232 - 2.500
R-factor	0.2137
Rwork	0.212
R-free	0.24670
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.232	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	12871	1250
<I/σ(I)>	23.8	1.45
Completeness [%]	100.0	100
Redundancy	14.1
CC(1/2)	0.500	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M sodium citrate, 5% propanol and 20% PEG 4000