6MZN
Zebrafish betaglycan orphan domain structure from tetragonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-14 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 |
Unit cell lengths | 62.940, 62.940, 114.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.330 - 2.380 |
R-factor | 0.202 |
Rwork | 0.198 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mzp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.110 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.080 | 1.784 |
Rmeas | 0.093 | 2.104 |
Rpim | 0.046 | 0.786 |
Number of reflections | 17750 | 1873 |
<I/σ(I)> | 10.6 | 1.1 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 7.5 | 6.8 |
CC(1/2) | 0.998 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8% v/v PEG 5K mono methyl ether, 4% w/v PEG 20K, 0.1M MES pH 6.5 |