6MZF
Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 218.800, 107.650, 282.740 |
Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
Resolution | 96.592 - 4.400 |
R-factor | 0.2331 |
Rwork | 0.225 |
R-free | 0.25150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.566 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 96.592 | 96.592 | 4.640 |
High resolution limit [Å] | 4.400 | 13.910 | 4.400 |
Rmerge | 0.061 | 0.478 | |
Rmeas | 0.198 | 0.069 | 0.675 |
Rpim | 0.112 | 0.038 | 0.386 |
Number of reflections | 80099 | 2576 | 11567 |
<I/σ(I)> | 4.9 | 6.1 | 1.4 |
Completeness [%] | 95.4 | 92.3 | 94.8 |
Redundancy | 2.9 | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000 |