6MYQ
Avian mitochondrial complex II with ferulenol bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.910, 84.553, 290.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.620 - 1.970 |
R-factor | 0.2088 |
Rwork | 0.208 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yq3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.013 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3150) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.620 | 2.030 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.085 | 0.905 |
Rmeas | 1.159 | |
Rpim | 0.710 | |
Number of reflections | 115414 | 7077 |
<I/σ(I)> | 5.7 | 0.9 |
Completeness [%] | 94.1 | 70.3 |
Redundancy | 3.22 | 2.04 |
CC(1/2) | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, crystal was soaked with ferulenol after crystallization |