6MYP
Avian mitochondrial complex II with TTFA (thenoyltrifluoroacetone) bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97430 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.951, 84.303, 290.194 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.360 - 2.100 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yq3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.510 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((DEV_3150: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.360 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.114 | 0.636 |
Number of reflections | 100834 | |
<I/σ(I)> | 6.9 | 2.2 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 4.7 | 4.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 48 g/L PEG3350, 15 mL/L PEG400, 24 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 1.6 mM manganese chloride, 0.64 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, TTFA |