6MYO

Avian mitochondrial complex II with flutolanyl bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978483
Spacegroup name	P 21 21 21
Unit cell lengths	69.637, 83.783, 289.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 2.200
R-factor	0.19
Rwork	0.189
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((DEV_3150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.650	2.240
High resolution limit [Å]	2.173	2.170
Rmerge	0.118	1.154
Number of reflections	80438
<I/σ(I)>	9.81	1.6
Completeness [%]	90.1	43.7
Redundancy	9.7	5.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside