6MYM

Crystal structure of hemagglutinin from influenza virus A/Phillipines/2/1982 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-16
Detector	NOIR-1
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	195.780, 187.580, 107.730
Unit cell angles	90.00, 109.82, 90.00

Refinement procedure

Resolution	48.580 - 2.450
R-factor	0.2283
Rwork	0.227
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp5
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.580	2.490
High resolution limit [Å]	2.450	2.450
Number of reflections	133433	6553
<I/σ(I)>	13.8
Completeness [%]	99.7	99.9
Redundancy	13.8	12.6
CC(1/2)	0.998	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris pH 8.5, 2.5M ammonium sulfate