6MYI

Pleurotus ostreatus OstreolysinA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	46.430, 100.335, 59.018
Unit cell angles	90.00, 106.43, 90.00

Refinement procedure

Resolution	33.305 - 1.150
R-factor	0.1419
Rwork	0.142
R-free	0.16060
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	1.154
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.120
High resolution limit [Å]	1.100	2.990	1.100
Rmerge	0.046	0.031	0.915
Rmeas	0.050	0.034	1.025
Rpim	0.019	0.013	0.449
Number of reflections	205258	10552	9347
<I/σ(I)>	7.2
Completeness [%]	97.8	99	89.6
Redundancy	6.2	6.8	4.4
CC(1/2)		0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M ammonium chloride, 0.05 M tris hydrochloride, 0.15 M sodium chloride, 19% (w/v) PEG3350, 30% (w/v) ethylene glycol